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Peptides can be modified at the N terminus, in the middle, or at the C terminus. In general, some standard peptide moieties must be accessible in order to be modified:
The purified PAL catalytic core PALcc contains molar equivalents of calcium and zinc along with substoichiometric amounts of iron and functions as a ureidoglycolate lyase. Limiting iron availability in the cells synthesizing PALcc reduces the specific activity of the enzyme produced.
This approach significantly reduced the number of spectra requiring interpretation, decreasing the computing time required for database searching and enabling manual interpretation of unidentified spectra. Amidation at the C-terminus is an important post-translational modification common to many neuropeptides Figure 1A.
Etomidate  USAN , INN , BAN marketed as Amidate is a short-acting intravenous anaesthetic agent used for the induction of general anaesthesia and sedation  for short procedures such as reduction of dislocated joints, tracheal intubation , and cardioversion. It was developed at Janssen Pharmaceutica in and was introduced as an intravenous agent in in Europe and in in the United States.
We report a case study of an IgG1 with a unique basic charge variant profile caused by C-terminal proline amidation on either one or two heavy chains. The proline amidation was sensitive to copper ion concentration in the production media during cell culture:
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